PRISM: Photon Resolved Image Scanning Microscopy for Spatial and Temporal Imaging of Living Samples

Fluorescence confocal laser-scanning microscopy (CLSM) is a cornerstone of life science research, renowned for its high-resolution imaging capabilities. This popularity is expected to grow with the advent of single-photon array detectors, which offer unique spatiotemporal information and open new perspectives for gentle and quantitative super-resolution imaging. Our innovative microscope, PRISM, transcends traditional CLSM limitations by employing a 49-element single-photon avalanche diode (SPAD) array, achieving unparalleled spatial and temporal super-resolution imaging. Central to PRISM is an advanced data-acquisition module that captures essential spatial and temporal photon information. This innovative solution has the potential to transform conventional fluorescence microscopes into intelligent, super-resolved systems, offering a new paradigm in microscopic imaging for living samples.